Effect of artificial cycle on expression of MMP-9 and TGF-β1 of endometria in patients with uterine adhesion
10.3969/j.issn.1671-8348.2014.02.014
- VernacularTitle:人工周期对宫腔粘连患者子宫内膜中MMP-9和 TGF-β1表达的影响
- Author:
Yingxin HE
;
Caixia TANG
;
Jue LIU
;
Meiqing LI
;
Chunhua XIE
- Publication Type:Journal Article
- Keywords:
estrogens;
ulterine diseases;
adhesions;
periodicity;
matrix metalloproteinase 9;
transforming growth factor beta1
- From:
Chongqing Medicine
2014;(2):167-169,172
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of the artificial cycle on the prognosis after transcervical resection of adhesion (TCRA) and its mechanism .Methods 80 patients with intrauterine adhesion were randomly divided into the observation group and the control group .The observation group were performed TRCA and postoperative artificial cycle for successive 3 months :oral es-tradiol valerate(9 mg once daily) for 21 d ,adding oral medroxyprofgesterone acetate(10 mg once dialy) on 15 d ,then the medication discontinuation for 7 d ,which was taken as 1 cycle;the control group received only TCRA .The re-examination was performed after 3 months .The levels of follicle stimulating hormone(FSH) ,luteinizing hormone(LH) ,estradiol(E2 ) ,prolactin(PRL) ,progesterone (P) and testosterone(T) were examined by ELISA after TCRA three months .The expression of MMP-9 and TGF-β1 in adhesive endometrium were detected by real-time PCR and Western blot .Results Compared with the control group ,the artificial cycle thera-py combined with the operation could significantly increase the effective rate of TCRA for treating mild and moderate intrauterine adhesion(P<0 .05);the seum levels of FSH ,LH ,E2 ,PRL ,P and T had no statistical differences between the observation group and the control group(P>0 .05);the artificial cycle significantly improved the adhesive degree of intrauterine adhesion ,increased the MMP-9 expression and decreased the TGF-β1 expression .Conclusion Artificial cycle could improve the degree of uterine cavity ad-hesion after TCRA ,which the mechanism might be related to the increase of MMP-9 expression and decrease of TGF-β1 expres-sion .
