Simultaneous Determination of Pinoresinol Diglucoside and Chlorogenic Acid in Traditional Decoction and Its Solid Decoction of Sam Soup by HPLC
10.3969/j.issn.1005-5304.2013.10.016
- VernacularTitle:RP-HPLC同时测定三圣汤及其固体汤剂中松脂醇二葡萄糖苷和绿原酸的含量
- Author:
Yuan LIN
;
Liangliang ZHOU
;
Yongdong LUO
- Publication Type:Journal Article
- Keywords:
Sam Soup;
solid decoction;
HPLC;
pinoresinol diglucoside;
chlorogenic acid
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2013;(10):44-45,46
- CountryChina
- Language:Chinese
-
Abstract:
Objection To estabish a HPLC methold for determining the contents of pinoresinol diglucoside and chlorogenic acid in Sam Soup and its solid decoction. Methods Chromatographic column was Ultimate XB-C18 reversed-phase chromatography column (250 mm×4.6 mm, 5 μm). Mobile phase was acetonitrile-0.4% glacial acetic acid with gradient elutim. The flow rate was 1.0 mL/min, detection wavelength was 277 nm, and column temperature was 40 ℃. Results The pinoresinol diglucoside and showed good linear relationship in the range of 5.0-50.0 μg, r =0.999 9 (n =6), and chlorogenic acid showed linear relationship in the range of 5.4-54.0 μg, r=0.999 6 (n=6). The average recoveries of pinoresinol diglucoside and chlorogenic acid were 98.75% (RSD=1.39%) and 101.11% (RSD=2.69%), respectively. Conclusion The established method was accurate and reliable. The contents of pinoresinol diglucoside and chlorogenic acid in solid decoction are higher than traditional decoction of Sam Soup.
