Extraction and Determination of Alkaloids from Bozhou Peony Paeonia lactiflora

VernacularTitle:亳芍生物碱提取及含量测定
Author: Naidong CHEN ; Fei ZHOU
Publication Type:Journal Article
Keywords: Bozhou Peony Paeonia lactiflora; alkaloids; color reaction; acid-base titration
From: Chinese Journal of Information on Traditional Chinese Medicine 2013;(10):49-50,51
CountryChina
Language:Chinese
Abstract: Objective To explore the extraction and assaying of the alkaloids from Bozhou Peony Paeonia lactiflora. Methods Dry root powder of the medical material was extracted by 90%ethanol. The extraction was first acidified by hydrochloric acid after recovering alcohol, and then extracted by dichloromethane. The acid solution was adjusted pH to alkalescence by ammonia water, then extracted by chloroform to get crude alkaloid. Bozhou Peony alkaloids were confirmed by TLC along with different colouration method, and its content was detected by acid-base titration. Results Four alkaloid-representative salmon patches were observed by colored with improving potassium heptaiodobismuthate method, and two prunosus blotches, four dark prunosus spots were delected by concentrated sulfuric acid and formaldehyde-concentrated sulfuric acid method, respectively. However, no chromogenic spots were found by colored with nitric acid as well with ninhydrin. The content of alkaloids in Bozhou Peony Paeonia lactiflora was about 0.079%. Conclusion Bozhou Peony Paeonia lactiflora contained alkaloid, and may including aconitum alkaloids.