POT1 Gene Expression in Patients of Acquired Aplastic Anemia with Different TCM Syndromes and Correlation Study
10.3969/j.issn.1005-5304.2013.08.007
- VernacularTitle:端粒保护蛋白1在不同证型获得性再生障碍性贫血患者中的表达及相关性研究
- Author:
Yan WANG
;
Ruirong XU
;
Siyuan CUI
;
Kui LIU
;
Jingyi WANG
- Publication Type:Journal Article
- Keywords:
acquired aplastic anemia;
TCM syndrome;
peripheral blood mononuclear cells;
POT1
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2013;(8):18-20,35
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the mRNA expression levels of POT1 in peripheral blood mononuclear cells of acquired aplastic anemia patients with different syndrome, and explore its relationship with acquired aplastic anemia and its TCM syndrome. Methods Peripheral blood mononuclear cells of 52 cases with acquired aplastic anemia and 20 cases as control group were collected to detect mRNA expression of POT1 by using real-time quantitative polymerase chain reaction (RT-qPCR), and its relation with TCM syndrome was analyzed. Results The expression levels of POT1 mRNA in patients with acquired aplastic anemia were lower significantly than control group (P<0.05). The expression levels of POT1 mRNA in patients with deficiency of kidney-yin were lower than patients with deficiency of kidney-yang, and it was lowest in patients with kidney deficiency of both yin and yang. There was significant correlation between the expression levels of POT1 mRNA and age (r=0.374, P=0.038). Conclusion The changes in expression levels of POT1 play a role in the pathogenesis of acquired aplastic anemia. There is correlation between mRNA expression level of POT1 in peripheral blood mononuclear cells and TCM syndrome.
