Effects of recombinant platelet-derived growth factor CC on resolution,organization and recanalization of venous thrombus
10.3969/j.issn.1671-8348.2013.24.022
- VernacularTitle:重组血小板衍生生长因子CC促静脉血栓溶解、机化、再通的实验研究
- Author:
Jiong LI
;
Meng YANG
;
Wenjun LUO
;
Bo TANG
;
Feng LI
;
Zhenyun JIN
- Publication Type:Journal Article
- Keywords:
platelet-derived growth factor CC;
venous thrombus;
resolution;
organization;
recanalization
- From:
Chongqing Medicine
2013;(24):2877-2880,2883
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study aimed to investigate whether platelet-derived growth factor CC could enhance resolution ,or-ganization and recanalization of venous thrombus .Methods The experimental models of deep vein thrombosis in rats were induced as previously described and modified .The rats were randomly divided into four groups according to the dose of PDGF-CC(Group A :0 ng/100 μL ;Group B :100 ng/100 μL ;Group C :200 ng/100 μL and Group D :500 ng/100 μL) .The samples were obtained at 7 days after operation and the expressions of protein VEGF were detected by Western blot analysis ,respectively .Immunohistochemi-cal staining was used to observe the types of the positive cells expressing vWF in thrombus sections .HE staining and Masson stai-ning were used to analyze the area of resolution and organization in venous thrombi .The capillary number was analyzed quantitative-ly by high-power microscope .The area of thrombus and collagen fiber in each section were measured and analyzed with image analy-sis software .Results Animal models of venous thrombogenesis were performed successfully .The data of Western blot analysis demonstrated that the expressions of VEGF protein were significantly increased in group C and D than group B and A ,which were no statistical significance between group C and group D at day 7 after operation .The positive cells of expressing vWF mostly located in the thrombus border and venous wall and the types of the positive cells by immunohistochemical staining were ECs .The resolu-tion rates ,organization rates and recanalization capillary numbers were significantly higher in group C and D than those in group B and A ,and those in group B were higher than those in group A .Conclusion PDGF-CC could enhance resolution ,organization and recanalization of vein thrombi effectively through therapeutic angiogenesis pathway ,which provides a novel strategy for gene thera-py of venous thromboembolism disease .
