The inverse PCR:good method for deletion breakpoint mapping
10.3969/j.issn.1671-8348.2013.23.023
- VernacularTitle:反向PCR：缺失断点定位好方法
- Author:
Mingyao WEN
;
Huaping XIE
- Publication Type:Journal Article
- Keywords:
breast cancer;
cell line,MCF-7;
deletion breakpoint mapping;
the inverse PCR
- From:
Chongqing Medicine
2013;(23):2760-2761,2764
- CountryChina
- Language:Chinese
-
Abstract:
Objective To map the deletion breakpoint of 9p21 in breast cell line MCF-7 use the inverse PCR .Methods After di-gestion ,ligation and PCR reaction ,the breakpoint was confirmed by sequencing .Results The deletion breakpoint started at chr9 :21819532 and ended at chr9 :21989622 with a small insertion of ACTGG ,which was consistent with the result confirmed by the long range PCR .Conclusion The inverse PCR is one good method for deletion breakpoint mapping and suitable for large sample size detection .
