Fusion expression,detection and application of HHV-8 antigen
10.3969/j.issn.1671-8348.2013.23.003
- VernacularTitle:人类疱疹病毒8抗原融合的构建及初步应用
- Author:
Na WANG
;
Hong LENG
;
Beizhan YAN
;
Yuanyuan WANG
;
Wenqiao ZANG
;
Min LI
;
Guoqiang ZHAO
- Publication Type:Journal Article
- Keywords:
HHV-8;
ORF59,ORF65,K8 .1;
fusion antigen;
fusion PCR
- From:
Chongqing Medicine
2013;(23):2703-2705,2709
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct the prokaryotic expression plasmid of HHV-8 fusion antigen for diagnosis of HHV-8 infec-tion .Methods The combined fragment ORF59 ,ORF65 and K8 .1 by fusion PCR was integrated into pQE-80L and transfected into E .coli DH5α.Fusion protein was induced to express by IPTG .SDS-PAGE and Western blot were employed to detect the fusion protein .Fusion protein was used to detect serum of blood donors .Results The combined plasmid pQE-80L-ORF59-ORF65-K8 .1 was constructed successfully after verifying by restriction enzyme digestion and sequencing .The fusion protein was about 24 KD and could be specific combined with HHV-8 positive serum .The fusion protein had the same result to detect HHV-8 with the HHV-8 ELISA kit .Conclusion Fusion protein we construct can be used as diagnosis antigen to detect HHV-8 of blood donors and common people .
