Simultaneous Determination of Ferulic Acid and Paeoniflorin in Ankong Zhongzi Wan by Dual Wavelength HPLC

VernacularTitle:双波长HPLC同时测定安坤种子丸中阿魏酸和芍药苷含量
Author: Xiaohua ZHANG ; Junru HU ; Qinguo MA ; Xiaoshuan LIU ; Xixiang LI
Publication Type:Journal Article
Keywords: Ankong Zhongzi Wan; ferulic acid; paeoniflorin; dual wavelength HPLC
From: Chinese Journal of Information on Traditional Chinese Medicine 2013;(9):55-56,57
CountryChina
Language:Chinese
Abstract: Objective To determine ferulic acid and paeoniflorin in Ankong Zhongzi Wan by HPLC under dual wavelength ultraviolet detection. Methods Ferulic acid and paeoniflorin were separated by Waters SymmetryShield-C18 column (4.6 mm × 250 mm, 5 μm) with gradient elution of acetonitrile-0.1%phosphoric acid as the mobile phase at a flow rate of 1.0 mL/min. The detection wavelength was 230 nm and 323 nm. Results The linear relationship of ferulic acid and paeoniflorin was good in the range of 0.058 2-0.582 4 μg (r=0.999 4) and 1.664-16.64 μg (r=0.999 6), and the average recovery rate was 97.77% (RSD=1.88%) and 98.84% (RSD=1.96%), respectively. Conclusion The method is accurate and quick for determining the two effective components in Ankong Zhongzi Wan, and can be used for its quality control.