Analysis on frequency of 9 bp deletion of mitochondrial DNA in Miao population in three geographical regions of Guizhou
10.3969/j.issn.1671-8348.2013.22.007
- VernacularTitle:贵州3个地域苗族人群mtDNA 9 bp序列缺失频率
- Author:
Chanjuan WANG
;
Huan WANG
- Publication Type:Journal Article
- Keywords:
mitochondrial DNA;
genetic polymorphism;
Miao ethnic group;
Guizhou
- From:
Chongqing Medicine
2013;(22):2586-2587,2590
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analysis the frequency of 9 bp deletion polymorphism of mitochondrial DNA(mt DNA)among Miao population in three geographical regions of Guizhou.Methods Polymerase chain reaction and polyacrylamide gel electrophoresis (PCR-PAGE)were used in the study of mtDNA 9 bp deletion polymorphism,DNA sequence was used to verify the results.Results Each group who come from Basa,Shajin,Longjie in Guizhou,the frequency of 9 bp deletion of mtDNA value as 19.15%(9/47), 42.5%(17/40)and 29.55%(13/43).Short polymorphic frequency difference was statistically significant between Basa and Shajin groups(P<0.05).Conclusion The frequencies of 9 bp deletion of mtDNA are relatively high in Miao ethnic group in Guizhou. There are certain differences of the delegation frequencies among these groups.
