Evaluation of modified ELISA method to detect Hepatitis B surface antigen in saliva specimens

VernacularTitle:ELISA检测唾液中乙型肝炎表面抗原方法的优化与评价
Author: Jin ZHANG
Publication Type:Journal Article
Keywords: HBsAg; saliva; ELISA
From: Chongqing Medicine 2013;(22):2611-2612,2615
CountryChina
Language:Chinese
Abstract: Objective To evaluated a modified ELISA method for detecting the Hepatitis B surface antigen(HBsAg)in saliva samples.Methods Optimized the different conditions,including sample type,sample volume and incubation condition,to screen the best condition.Three different methods to calculate cut-off value was evaluated to screen the most acceptable.Results The non-processed saliva sample can be used directly for detection(P=0.100).Test results of different sample volume was no significant difference(P=0.070).HBsAg was detected in 44 saliva samples out of 47 paired positive serum specimens and not detected in 63 saliva samples out of 68 matched negative serum samples by the optimized ELISA assay.There was excellent agreement between the results for the serum and saliva specimens and the kappa value was 0.87 for saliva specimens.Using an optimized protocol,the sensitivities and specificities were 93.6% and 92.6%,respectively.Conclusion Our data showed a significant promise for the use of the modified commercial ELISA in saliva sample for Hepatitis B virus infection surveillance.