The expression of sialic acid-binding immunoglobulin-like lectin-1 on peripheral blood mononuclear cells and its relationship with disease activity in rheumatoid arthritis
10.3760/cma.j.issn.1007-7480.2013.04.002
- VernacularTitle:类风湿关节炎患者外周血单个核细胞唾液酸结合免疫球蛋白样凝集素-1的表达及与疾病活动度的关系
- Author:
Yisong XIONG
;
Yue CHENG
;
Ailin WU
;
Yanyan WANG
;
Jie XIONG
;
Renqian ZHONG
- Publication Type:Journal Article
- Keywords:
Arthritis,rheumatoid;
Flow cytometry;
Membrane glycoproteins;
Immunoglobulins;
Monocytes
- From:
Chinese Journal of Rheumatology
2013;17(4):221-224,后插1
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of sialic acid-binding immunoglobulin-like lectin-1 (Siglec-1) in the peripheral blood mononuclear cells (PBMCs) in patients with rheumatoid arthritis (RA),osteoarthritis (OA) and healthy controls and to explore the relationship between Siglec-1 expression and disease activity in RA.Methods Siglec-1 protein and mRNA levels were measured by flow cytometry and real-time quantitative reversetranscription-polymerase chain reaction (qRT-PCR) in 42 RA patients,28 OA patients and 26 healthy controls,respectively.The correlation studies between Siglec-1 and disease activity score 28 (DAS28) or C-reactive protein were performed.T-test was used for comparisons between groups and Pearson's correlation test was used for correlation analysis.Results The percentage of Siglec-1 positive cells of PBMCs in RA group [(15.2±7.6)％] was significantly higher than that in the OA group [(2.3 ±2.6)％] or healthy controls [(2.1±1.6)％,t=8.615,8.661; all P＜0.01].And the major cell type in PBMCs that expressed Siglec-1 was monocytes.The relative Siglec-1 mRNA expression in PBMCs in the RA group (3.4±1.5) was also significantly higher than that in the OA group (1.2±0.4) or healthy controls [(1.0± 0.4),t=3.446,3.966; all P＜0.05].But no significant differences of Siglec-1 protein and mRNA between the OA group and healthy controls were found.Furthermore,positive correlations between Siglec-1 protein and DAS28 or hs-CRP were found in RA patients (r=0.89,P＜0.01; r=0.48,P＜0.01).Conclusion PBMCs are activated which are characterized by elevated expression of Siglec-1 in RA patients.Circulating Siglec-1 may be considered as a potential noninvasive biomarker for monitoring disease activity in RA.
