Expression of EZH2 mRNA and protein in human prostate carcinoma and its relationship with cell proliferation
10.3969/j.issn.1001-7399.2009.06.012
- VernacularTitle:前列腺癌中EZH2 mRNA及蛋白表达与细胞增殖的关系
- Author:
Jiang LI
;
Qinhe FAN
;
Xiangshan FAN
;
Wei ZHOU
;
Yan QIU
;
Liang QIU
- Publication Type:Journal Article
- Keywords:
prostate neoplasms;
EZH2;
tissue microarray;
immuohitochemistry;
in situ hybridization;
proliferation
- From:
Chinese Journal of Clinical and Experimental Pathology
2009;(6):615-618,623
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the expression of EZH2 mRNA and protein, and its relationship with tumor cell proliferation in human prostate carcinoma (PCa).Methods A tissue microarray was constructed, which contained 68 dots of formalin-fixed,paraffin-embedded tissue samples, including 48 cases of human PCa.Immunohistochemical markers, including EZH2 and Ki-67,were used on the tissue microarray sections by the immunohistochemical staining method.In situ hybridization (ISH) using an EZH2 oligonucleotide probe was also performed on the tissue microarray sections.Additional 15 cases of benign prostate hyperplasia (BPH) and 12 cases of high grade prostate intraepithelial neoplasia (HGPIN) were used as controls.Results The positive rates of EZH2 protein and mRNA expression were 87.50% and 81.25% in the PCa, 16.67% and 16.67% in the HGPIN,13.33% and 6.67% in the BPH,respectively. There were statistical difference between PCa, HGPIN and BPH, respectively (P<0.05).The expression of EZH2 protein and mRNA had no statistical difference (P>0.05).The expression of EZH2 protein was related to Gleason score, TNM stage (P<0.05), but not to age and serum prostate-specific antigen (PSA) level (P>0.05). The expression of EZH2 mRNA was related to TNM stage (P<0.05),but not to age, PSA and Gleason score (P>0.05).The staining intensity of EZH2 was positively correlated with the Ki-67 indexes (r=0.746,P<0.05).Conclusions Over-expression of EZH2 which is involved in accelerating proliferation may play an important role in the pathogenesis and progression of human prostate carcinoma. As a molecular marker of prostate carcinoma, EZH2 may serve as a new index for estimating the level of malignancy and progression of the prostate cancer.
