Identification of the gene differentially expressed in female Anopheles anthropophagus and cloning of its full-length cDNA

VernacularTitle:嗜人按蚊雌蚊差异表达基因的鉴定及全长cDNA的克隆
Author: Yonggang YE ; Yirui ZHAO ; Renli ZHANG ; Yijie GENG ; Dana HUANG ; Shitong GAO ; Ruiqing LIN ; Xingquan ZHU
Publication Type:Journal Article
Keywords: Anopheles anthropophagus; gene specially expressed in femal; real-time PCR; RACE
From: Chinese Journal of Zoonoses 2009;(12):1177-1180,1185
CountryChina
Language:Chinese
Abstract: To identify the gene differentially expressed in female Anopheles anthropophagus and to analyze its gene sequence, this gene amplified by PCR was identified by real-time PCR and its cDNA was then amplified with rapid amplification of cDNA ends (RACE) technology. It was found that the expression ratio of the female differentially expressed gene in female and male mosquitoes was 267.49 according to the formula F=2~(-⊿⊿CT).The size of mRNA of the gene was 364 bp, and the amino acid sequence deduced from the open reading frame (ORF) was found to be similar to the sequence of tectin protein of Culex quinquefasciatus and proteins of other species. The mRNA sequence of this gene was submitted to NCBI with a accession number of FJ907236. This gene may provide a foundation for further studies on the biological functions of mosquitoes.