The effect of simultaneously blocking target epidermal growth factor receptortyrosine kinase and cyclooxygenase-2 on the growth of NPC cell
10.3969/j.issn.1001-1781.2009.18.001
- VernacularTitle:靶向联合阻断表皮生长因子受体和环氧化酶-2信号通路影响鼻咽癌细胞生长的研究
- Author:
Shisheng LI
;
Xin ZHANG
;
Jing XU
;
Li XIE
;
Yuanzheng QIU
;
Xin SHU
;
Yongquan TIAN
- Publication Type:Journal Article
- Keywords:
nasopharyngeal neoplasms;
epidermal growth factor receptor;
cyclooxygenase-2;
combined targeting therapy
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2009;(18):817-820,823
- CountryChina
- Language:Chinese
-
Abstract:
This study was designed to prove simultaneously blocking both EGFR and COX-2-medi-ated pathways may be an efficient means of inhibiting cancer cell growth in NPC. Method: A combination of tarceva (EGFR-selectivetyrosine kinase inhibitors) with celecoxib(Cox-2 inhibitor) was studied on its effects on cell growth, cell cycle progression, apoptosis and protein expression in CNE-2 cell lines by cell growth assay, flow cy-tometric analysis assay and Western blotting. Result: ①The inhibition rate of cell growth was higher in the group treated with a combination of two agents than that the sum of rates of the two groups treated with only one agent (P<0.05). ②The combination of tarceva with celecoxib significantly induced G_1 arrest(P<0.05) ,but did not in-crease apoptosis rate(P>0.05). ③The group of combination showed less expressions of p-EGFR and COX-2 than any other group. Conclusion Simultaneously blocking EGFR and COX-2 mediated pathways would significantly in-hibit the growth of CNE-2 cell line, increase G_1 arrest and reduce the expression levels of p-EGFR and COX-2.
