The relationship of the difference of sol precursor protein expression and chemotherapy drug resistance in breast cancer
10.3760/cma.j.issn.1008-6706.2013.14.002
- VernacularTitle:凝溶胶蛋白前体的差异表达与乳腺癌化疗耐药性的相关性研究
- Author:
Ping SUN
;
Aihong JIAO
;
Liangming ZHANG
- Publication Type:Journal Article
- Keywords:
Proteomics;
Breast neoplasms;
Antineoplastic combined chemotherapy protocols;
Multidrug resistance-associated proteins
- From:
Chinese Journal of Primary Medicine and Pharmacy
2013;20(14):2083-2085,后插1
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the change of precursor protein of sol in breast cancer before and after the neoadjuvant chemotherapy,so as to find the serum protein marker of chemotherapy drug resistance in breast cancer.Methods Breast cancer patients with Ⅲ period neoadjuvant chemotherapy were enrolled in the study.The serum specimens of the patients were obtained.Two-way gel electrophoresis was used to get the gel map before and after chemotherapy,then the image map was analyzed by gel analysis software.The differentially expressed proteins point was screened before and after chemotherapy.Serum protein differences were identified with matrix auxiliary laser disi ntegrate series time of flight mass spectrometer (MALDI-TOF-MS),including coagulation sol precursor protein.Western blot was used to test the variation trend of them before and after chemotherapy.Results Coagulation sol precursor protein levels fell after neoadjuvant chemotherapy in resistance group.The variation trend of the results of western blot and that of the proteomics were consistent.Conclusion Coagulation sol precursor protein can be as the candidate serum marker of chemotherapy drug resistance in breast cancer.
