Determination of baicalin in Fushuye by HPLC
- VernacularTitle:HPLC测定妇舒液中黄芩苷含量
- Author:
Yonglin WANG
;
Yanyu LAN
;
Baozhu ZHOU
- Publication Type:Journal Article
- Keywords:
Fushuye;
baicalin;
HPLC
- From:
Chinese Pharmaceutical Journal
1999;(4):265-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To domminate the quality of Fushuye,the contents of baicalin in Fushuye was determined by HPLC.METHOD:Baicalin was separated on Spherisorb C18 column and detected at 278 nm,by using methanol-4g.L-1 phosphoric acid (50∶50) as mobile phase.RESULTS:The resolution was 4.5.The number of theoretical plates calculaled for baicalin peak was 3800.The standard curve was linear over the range of 20 ng～900ng,with the correlation coefficient 0.999 9.The average recovery was 99.88% with RSD=1.54%(n=5). CONCLUSION:The method is simple and fast,and can be used for quantitative analysis of Fushuye.
