Comparison of the relative content of trichosanthin between root and callus derived from Trichosanthes kirilowii
- VernacularTitle:栝楼愈伤组织和块根中天花粉蛋白相对含量的比较研究
- Author:
Xiwen LI
;
Hongxia TONG
;
Qingshan LI
- Publication Type:Journal Article
- Keywords:
Trichosanthes kirilowii Maxim.,calli of the leaves;
trichosanthin (TCS);
immuno-precipitation reaction;
electrophoregram scanning
- From:
Chinese Pharmaceutical Journal
1999;(2):83-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：The relative content of trichosanthin (TCS) of the calli induced from the leaves of Trichosanthes kirilowii Maxim. was measured and a comparison between the calli and the root was made. METHODS：TCS was obtained by the fractional precipitate with acetone from the homogenate of the root or the calli. To examine and measure TCS, several methods, such as immuno-precipitation reaction, SDS-PAGE and electrophoregram scanning, were usde. RESULTS：The results of immuno-precipitation reaction and SDS-PAGE showde that TCS existed in the calli and in the root of T.kirilowii Maxim.. It was found that TCS was the richest component in the acetone precipitated crude extract of the calli with a relative content of 44.22% TCS in the extract, though the absolute content of TCS in the calli was less than that in the root. CONCLUSION:Extracting TCS from the calli derived from leaves has not been reported previously. The absolute content of TCS in the root is 2.66 times more than that in the calli.
