Determination of puerarin in Xinning capsule by HPLC assay
- VernacularTitle:HPLC测定心宁胶囊中葛根素含量
- Author:
Yonglin WANG
;
Ling TAO
;
Baozhu ZHOU
- Publication Type:Journal Article
- Keywords:
Xinning capsule;
puerarin;
HPLC
- From:
Chinese Pharmaceutical Journal
1999;(3):187-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To domminate the quality of Xinning capsule,the contents of Puerarin in Xinning capsule was determined by HPLC.METHOD:Puerarin was separated on Spherisorb C18 column and detected at 250nm,by using methanol-water-phosphoric acid (280∶720∶0.15)as mobile phase.RESULTS:The resolution was greater than 1. The number of theoretical plates calculaled for Puerarin peak was 8310;The standard curve was linear over the range of 0.2～2μg with the correllation coefficient 0.9999.The average recovery was 99.4% with RSD=2.47%(n=7).CONCLUSION:The method is simple and fast,and can be used for quantitative analysis of Xinning capsule.
