Polarization of protective immunity induced by replication-incompetent adenovirus expressing glycoproteins of pseudorabies virus.
10.3858/emm.2008.40.6.583
- Author:
Young Woo HAN
1
;
Abi G ALEYAS
;
Junu A GEORGE
;
Seon Ju KIM
;
Hye Kyung KIM
;
Hyun A YOON
;
Dong Jin YOO
;
Seong Ho KANG
;
Koanhoi KIM
;
Seong Kug EO
Author Information
1. Laboratory of Microbiology, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, Jeonju, Korea. vetvirus@chonbuk.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
adenoviruses, human;
herpesvirus 1, Suid;
Th1 cells;
Th2 cells;
vaccination
- MeSH:
Adenoviridae/genetics/*immunology/metabolism;
Animals;
Antibody Formation;
Cell Line;
Cytokines/immunology;
Female;
Glycoproteins/biosynthesis/genetics/*immunology;
Herpesvirus 1, Suid/genetics/*immunology/physiology;
Immunity, Cellular;
Immunoglobulin G/immunology;
Mice;
Mice, Inbred C57BL;
Pseudorabies/*immunology/prevention & control;
Pseudorabies Vaccines/administration & dosage/*immunology;
Swine;
Th1 Cells/immunology;
Th2 Cells/immunology;
*Virus Replication
- From:Experimental & Molecular Medicine
2008;40(6):583-595
- CountryRepublic of Korea
- Language:English
-
Abstract:
Replication-incompetent adenoviruses expressing three major glycoproteins (gB, gC, and gD) of pseudorabies virus (PrV) were constructed and used to examine the ability of these glycoproteins to induce protective immunity against a lethal challenge. Among three constructs, recombinant adenovirus expressing gB (rAd-gB) was found to induce the most potent immunity biased to Th1-type, as determined by the IgG isotype ratio and the profile of the Th1/Th2 cytokine production. Conversely, the gC-expressing adenovirus (rAd-gC) revealed Th2-type immunity and the gD-expressing adenovirus (rAd-gD) induced lower levels of IFN-gamma and IL-4 production than other constructs, except IL-2 production. Mucosal delivery of rAd-gB induced mucosal IgA and serum IgG responses and biased toward Th2-type immune responses. However, these effects were not observed in response to systemic delivery of rAd-gB. In addition, rAd-gB appeared to induce effective protective immunity against a virulent viral infection, regardless of whether it was administered via the muscular or systemic route. These results suggest that administration of replication-incompetent adenoviruses can induce different types of immunity depending on the expressed antigen and that recombinant adenoviruses expressing gB induced the most potent Th1-biased humoral and cellular immunity and provided effective protection against PrV infection.
