Isolation and identification of pathogenic bacterium which caused hemorrhagic diarrhea in newborn swine
10.3969/j.issn.1002-2694.2010.03.004
- VernacularTitle:一株致仔猪腹泻大肠杆菌的分离与鉴定
- Author:
Hongtao JIN
;
Zhiping XIA
;
Dan LI
;
Huiliang XUE
;
Jun LIU
;
Hai LIAN
- Publication Type:Journal Article
- Keywords:
diarrhea;
Escherichia coli;
toxicity gene;
H10
- From:
Chinese Journal of Zoonoses
2010;(3):209-210,214
- CountryChina
- Language:Chinese
-
Abstract:
The Escherichia coli (E.coli) strain from intestinal tract of died newborn swine was isolated and cultured.Preliminary identification of the isolated strain was conducted by conventional biochemical tests,and the molecular biology detections of toxicity gene and typing gene were completed by multi-PCR.Stable toxin and heat-labile enterotoxin genes of E.coli were detected from the isolated strain.By amplifying and sequencing bacterial 16s rDNA and fliC gene,the isolated strain was identified as H10 by Blast analysis.The homology of strain H10 was 99% with bacterial 16s rDNA gene and 98% with fliC gene.
