Analysis of the fatty acid components of Brucella strains in Guangdong province
10.3969/j.issn.1002-2694.2010.02.009
- VernacularTitle:广东省布鲁氏菌菌株脂肪酸成分分析
- Author:
Jingdiao CHEN
;
Xiaoling DENG
;
Changwen KE
;
Buyun CUI
;
Bixia KE
;
Meizhen LIU
;
Hailing TAN
;
Bosheng LI
- Publication Type:Journal Article
- Keywords:
Brucella;
fatty acids;
analytical method
- From:
Chinese Journal of Zoonoses
2010;(2):131-133,139
- CountryChina
- Language:Chinese
-
Abstract:
To explore the possibility to type the Brucella strains isolated in Guangdong province with analytical method to detect the fatty acid components and to collect the basic data of fatty acid components of Brucella strains, 29 strains of Brucella were selected for analysis on the bacterial fatty acid components and the cluster analysis on the collected data was performed with Sherlock analysis soft-ware (MIDI). It was demonstrated that the main fatty acid components of Brucella strains isolated in Guangdong province were 19∶0 cycloω8c acid, 16∶0 acid and 18∶0 acid. The content of 19∶0cycloω8c acid was highest in B.abortus, followed by B.melitensis and lowest in B.suis.-In addition, the content differences of 19∶0cycloω8c and 18∶0 acid between B. melitensis and Brucella suis were statistically significant; and that of 19∶0cycloω8c and 18∶0 acid between strains isolated in 1965 and those isolated in recent 3 years was statistically significant. It was also shown that the fatty acid components of Brucella strains were stable, but the contents of fatty acid components were different in different species.-It is evident that at certain euclidean distance, 3 species of Brucella can be differentiated in species level.
