Effects of various pH in testing bacterial endotoxins
- VernacularTitle:pH值影响细菌内毒素测定的实验室研究
- Author:
Guozheng GAO
;
Jin YAN
- Publication Type:Journal Article
- Keywords:
bacterial endotoxin;
pH;
tachypleus amebocyte lysate;
limulus amebocyte lysate;
kenitic-turbidimetry
- From:
Chinese Pharmaceutical Journal
1998;(3):163-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To study the effect of samples having different pH values in the bacterial endotoxins test (BET). METHODS: The BET was conducted as an assay of the concentration of endotoxins by using kinetic-turbidimetric technique. Samples having different pH values but containing same concentration reference standard endotoxin (RSE) were tested by TAL/LAL reagents. The average result on the standard curve was calculated and the mean recovery observed. RESULTS: The reagents had three highly sensitivities in pH 5.20, pH 7.83/pH 6.23 and pH 10.55. After adjustment to pH 6 to 8, the mean recovery was 76.5%～115.9% or 85.8%～99.8%. When the sample pH value was less than 3 or more than 12, the test was unsuitable for the inhibition test, and the mean recovery was less than 56.8%. CONCLUSION: It is necessary to adjust the pH of the solution to be tested in the BET, adjust the sample to pH (6.5～7.5) when using TAL reagents, and adjust to pH (6.2±0.1) or pH(6.7～7.8) when using LAL reagents.
