Primary Culture and Identification of Atrial Myocytes of Neonatal Rat
- VernacularTitle:新生大鼠心房肌细胞的培养与鉴定
- Author:
Xiaodan FU
;
Bo YU
;
Yang LI
;
Liang MENG
;
Zhenhuan JIANG
;
Ying YANG
- Publication Type:Journal Article
- Keywords:
cardiomyocytes;
primary culture;
neonatal rat
- From:
Journal of China Medical University
2010;(9):717-718,723
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method to culture and isolate atrial myocardial cells of neonate rat. Methods The atrial myocardial tissue was digested by a low concentration of trypsin and eollagenase. Subsequently, the atrial myocardial cells were cultured and isolated with the technique of differential anchoring for twice. The purity of cardiocytes was detected by immunofluorescenee and the pulsation was observed under inverted biologicalmicroscope. Results After 72 hours of primary culture, the cells reached 70% confluence, and all the my- ocardial cells were jumping synchronously. Immunofluorescence showed that the purity of cardiocytes cultured was more than 90%. Conclu- sion Neonatal rat atrial myocytes were successfully cultured by enzyme digestion with high purity and vigor.
