Promoting Effect of Schwann＇s Cells on the Differentiation of Rat Bone Marrow-derived Mesenchymal Stem Cells into Neuron-like Cells
- VernacularTitle:雪旺细胞促进大鼠骨髓间充质干细胞分化为神经元样细胞的效应研究
- Author:
Xiaodong ZHI
;
Gang LU
- Publication Type:Journal Article
- Keywords:
Sehwann＇s cells;
transdiffereciafion;
bone marrow-derived mesenehymal stem cells;
neuron-like cells
- From:
Journal of China Medical University
2010;(9):737-739,742
- CountryChina
- Language:Chinese
-
Abstract:
Objective To approach a new method induce the differentiation of mesenchymal stems cells（MSC）into neuron-like cells.Methods We co-cultured Schwann cells with MSC to induce the differentiation of rat MSC into neuron-like cells.Immunohistochemistry was employed to observe the expression of NSE and Brdu,and Western blot to examine the expression of NeuN by in transdifferenciated MSC（Neuron-like cells）.Additionally,we analyzed the differentiating rate of MSC.Results The co-culture of MSC with Schwann cells could induce the expression of NSE and NeuN.They have been differentiated into neuron-like cells.The differentiating rate of MSC was（80.51±5.65）%.Conclusion The co-culture with Schwann′s cells could differentiate rat bone marrow derived MSC into Neuron-like cells.
