Chemokine Receptor 5 of Human Brain Microvascular Endothelial Cells Takes Part in Amyloid beta Induced T Lymphocytes Transendothelial Migration
- VernacularTitle:脑内皮细胞趋化因子受体5参与β淀粉样蛋白引起的T细胞跨内皮迁移
- Author:
Li ZHU
;
Man LI
;
Weidong ZHAO
;
Yuhua CHEN
- Publication Type:Journal Article
- Keywords:
amyloid beta;
chemokine receptor 5;
brain microvascular endothelial cell;
T lymphocytes;
transendothelial migration
- From:
Journal of China Medical University
2010;(6):410-412,431
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the function of chemokine receptor 5（CCR5）in human brain microvascular endothelial cells（HBMEC）during T lymphocytes transendothelial migration induced by amyloid beta（Aβ）.Methods The in vitro model of blood-brain barrier was established with cultured HBMEC monolayer.The ability of T lymphocytes transendothelial migration induced by Aβ was analyzed by Transwell and Millicell-ERS endothelia volt-ohmmeter.Results Aβ could promote T lymphocytes to migrate through the HBMEC monolayer.Over-expression of CCR5 in HBMEC promoted T lymphocytes to migrate through HBMEC monolayer.Expression of CCR5 mutant in HBMEC inhibited the transendothelial migration of T lymphocytes.Conclusion CCR5 of HBMEC is involved in T lymphocytes transendothelial migration induced by Aβ.
