Neuroprotective Effect of Celecoxib against Degeneration of Dopaminergic Neurons in Rat Model of Parkinson Disease
- VernacularTitle:塞来昔布对脂多糖所致PD大鼠黑质多巴胺能神经元损伤的保护作用
- Author:
Ping NIU
;
Xin CHEN
;
Congjie WANG
;
Baoru LIU
;
Zhiqiang XIN
- Publication Type:Journal Article
- Keywords:
celecoxib;
dopaminergic neuron;
lipopolysaccharide;
neuroinflammation
- From:
Journal of China Medical University
2010;(3):191-193,204
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the neuroprotective effect of celecoxib against degeneration of dopaminergic neurons caused by lipopolysaccharide in vivo.Methods The rat model of Parkinson disease(PD)was established by intranigral injection of lipopolysaccharide.Sprague-Dawley rats were randomly divided into control group,PD group,and celecoxib group.Behavioural changes were recorded,and the expressions of tyrosine hydroxylase(TH)and cyclooxygenase-2(COX-2)were determined by immunohistochmistry and Western blot.Results No behavioral change was found in control group.There was significant difference in the number of circling behavior between PD and celecoxib groups(196.90±9.52 vs 109.30±9.38,P＜0.01).The number of TH-positive cells and the expression of TH protein in rat substantia nigra were significantly higher in celecoxib group than in PD group(P＜0.01).Compared with PD group,the number of COX-2positive cells and the expression of COX-2 protein were significant lower in celecoxib group(P＜0.01).Conclusion Celecoxib has neuroprotective effect on the degeneration of dopaminergic neurons caused by lipopolysaccharide in vivo.
