Effects of Transcutaneous Electrical Acupoint Stimulation on the Plasma Amino Acid and Extracellular 5-HT in Dorsal Raphe Nucleus of Exercising Rats
- VernacularTitle:经皮穴位电刺激对跑台运动大鼠血浆氨基酸及中缝背核细胞外液5-HT含量的影响
- Author:
Jianqiao FANG
;
Yi LIANG
;
Cunxin WANG
;
Xiaomei SHAO
;
Guizhi MA
;
Haiyan LI
- Publication Type:Journal Article
- Keywords:
transcutanous electrical acupoint stimulation;
treadmill running;
microdialysis;
tryptophan;
serotonin
- From:
Chinese Journal of Sports Medicine
2010;(3):309-312,320
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of transcutanous electrical acupoint stimulation(TEAS)on the exercise ability of rats.Methods After stereotaxis surgery,twenty male adult SD rats wera randomly divided into control group (n=10)and TEAS group(n=10).Two days after surgery.the rats were trained to adapt the treadmill mnning and TEAS(continuous wave,2Hz,5mA)was applied to right Zusanli (ST 36) for 30min,once a day for 6 days.Basic microdialysis samples of extracellular serotonin(5-HT)in rats'dorsal raphe nucleus(DRN)were collected 7 days after surgery.During experiment,rats were running on treadmill (10m/min for 10min,then gradually increased to 24m/min for 1 hour,0% grade).Microdialysis samples were collected continuously until 5 hours after treadmill running.The frequency of brush-stimulation during the running was recorded.Meanwhile,plasma amino acid content was examined 5 hours after exercise.Results The frequencies of brush-stimulation during the treadmill running in control group and TEAS group were 2.04+0.46 and 0.40+0.080 times per minute,respectively (P<0.05).There were no significant differences in plasma total-TRP(T-TPP)and branched chain amino acid(BCAA)contents between two groups(P>0.05).However,plasma free-TRP(f-TRP)content,f-TRP/T-TRP ratio and f-TRP/BCAA ratio decreased significantly(P<0.05) in TEAS group as compared with the control group.Extracellular 5-HT levels in DRN in control group soon after exercise were higher than their base levels(P<0.05),and decreased in sequential two hours,and then increased significantly 3 hours and 4 hours after exercise as compared with that 2 hours after exercise(P<0.05).There was a slow decline in the levels of extracellular 5-HT in TEAS group at each time-point after exercise,and significant lower than that in control group within 5 hours after exercise(P<0.05).Conclusion TEAS atZusanli(ST36)can effectively improve exercised ability by reducing peripheral free-TRP level and transporting TRP into the brain,and thus decreasing the synthesis of 5-HT in the brain.
