The Dynamical Changes of Serum β-APP in Early Stage of Rabbit Traumatic Brain Injury
- VernacularTitle:兔脑创伤后早期血清β淀粉样前体蛋白动态变化的实验研究
- Author:
Haineng HUANG
;
Qisheng LUO
;
Bang ZHAO
;
Yuanfu TAN
;
Yuanyang DENG
;
Huadong HUANG
;
Huangde FU
;
Chuanyu LI
- Publication Type:Journal Article
- Keywords:
traumatic brain injury;
enzyme linked immunosorbent assay;
β-amyloid precursor protein
- From:
Journal of China Medical University
2010;(1):22-23,30
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship of serum levels of β-amyloid precursor protein (β-APP) with degree of traumatic brain injury (TBI) and the traumatic time.Methods Sprague-Dawley (SD) rats were randomly divided into normal control and injury group.The rats in injury groups suffered from TBI after free-falling percussion with different pressure (wild-injury,moderate-injury and severe-injury group).Then serum was collected at 0.5 h,2 h,6 h,and 24 h and subject to β-APP detection by ELISA.All data were analyzed statistically with completely randomized design multiple factor repeated measure of variance analysis and least significant difference (LSD) test.Results The serum levels of β-APP were higher after injury.The serum levels of β-APP were significantly higher in moderate-injury or severe-injury group than those in normal group or slight-injury group (P＜0.05).The serum levels of β-APP were higher in severe-injury group than that in moderate-injury group with no statistical difference (P＞0.05).There was no statistical difference in serum β-APP levels between normal control and slight-injury group (P＞0.05).Conclusion The serum level of P-APP is increasingly higher with traumatic brain injury more serious and could be employed as an indicator of TBI degree.It implies that β-APP has the potential as an early diagnosis marker for TBI.
