The effect of hyperoxia on fluid transport by fetal distal lung epithelia
10.3760/cma.j.issn.1673-4408.2013.02.028
- VernacularTitle:高氧对胎鼠远端肺上皮细胞液体转运功能及上皮钠通道表达的影响
- Author:
Weihua JI
;
Jianhua FU
;
Xindong XUE
- Publication Type:Journal Article
- Keywords:
Hyperoxia;
Fetal distal lung epithelia;
Fluid transport;
Epithelial sodium channel
- From:
International Journal of Pediatrics
2013;(2):201-203,207
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of hyperoxia on fluid transport by fetal distal lung epithelia(FDLE) and the expression of epithelial sodium channel(ENaC) in these cells.Methods FDLE were isolated and randomized into hyperoxia group and normoxia group,which were primarily cultured under hyperoxic or normoxic conditions,respectively.Fluid transport was measured using monolayers of FDLE cultured on Transwell permeable inserts.Western blot was applied to examine the α-ENaC expression.Results Fluid transport across monolayer of FDLE was increased in cells exposed to hyperoxia compaired with cells cultured in normoxic conditions (1.78 ± 0.19 vs 1.06 ± 0.11,P ＜ 0.001).Amiloride significantly decreased the fluid transport in both of the hyperoxia and normoxia groups,but in the presence of amiloride there were no difference between the two groups.The expression of α-ENaC was inhibited by hyperoxia to some extent(24h:0.44 ±0.04 vs 0.40 ±0.04,P=0.22; 48h:0.35 ±0.03 vs 0.47 ±0.06,P =0.03).Conclusion Hyperoxia enhanced total and amiloride-sensitive fluid transport by FDLE.However,the expression of α-ENaC decreased in these cells.
