Up-regulation of calreticulin expression and its significance in patients with rheumatoid arthritis in vivo
10.3760/cma.j.issn.1007-7480.2013.01.007
- VernacularTitle:钙网织蛋白在类风湿关节炎患者体内表达上调及其意义
- Author:
Yichao WANG
;
Wei WEI
;
Chunyou WAN
;
Min NI
;
Na ZHANG
;
Hongyi LIU
;
Fang ZHENG
- Publication Type:Journal Article
- Keywords:
Arthritis,rheumatoid;
Synovial membrane;
Calreticulin
- From:
Chinese Journal of Rheumatology
2013;(1):27-30,封3
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of calreticulin (CRT) in the pathogenesis of rheumatoid arthritis (RA) by analyzing its expression in the sera,synovial fluid and synovial membrane in patients with RA.Methods Levels of CRT in the sera from patients with RA,osteoarthritis (OA),systemic lupus erythematosus (SLE),other autoimmune diseases and health control (HC) were detected by enzyme linked immunosorbent assay (ELISA) and Western bloting.CRT levels in synovial fluid from RA and OA patients were measured by ELISA.Pathological methods were employed to analyze the expression and localization of CRT in synovial membrane.ANVOA,SNK-q test were used for statistical analysis.Results CRT was found to be significantly up-regulated in sera from RA [(4.8±2.4) ng/ml] than that from in OA [(3.6±0.9)ng/ml],SLE [(4.0±1.5) ng/ml],other autoimmune diseases [(3.9±0.8) ng/ml] and HC [(3.7±0.6) ng/ml].Only the monomer form of CRT in the serum could be detected.Pathological results showed that in RA synovium,CRT was mainly expressed in the lining and sublining layers,endothelial cells and perivascular areas； while in OA,only few CRT staining was seen in perivascular areas and the synovial lines.Conclusion In RA,increased levels of CRT are detected in the sera.In addition,high expression of CRT is observed in synovium and its distribution are different from OA.Our results suggest that CRT may be involved in RA joint inflammation and pannus formation.CRT may become a potential serological marker in the diagnosis of RA.
