Quantitative determination of Hantavirus mRNA with multiple TaqMan-MGB fluorescence quantitative RTPCR and its value in clinical diagnosis
10.3760/cma.j.issn.1008-6706.2012.22.004
- VernacularTitle:肾综合征出血热多重TaqMan-MGB荧光定量RT-PCR快速检测方法的建立
- Author:
Li KANG
;
Hongbo QIAN
;
Rongfang XU
;
Li LIU
;
Yanping LI
- Publication Type:Journal Article
- Keywords:
Kidney syndrome hemorrhagic fever;
Hantavirus;
Polymerase chain reaction;
TaqManTM probe
- From:
Chinese Journal of Primary Medicine and Pharmacy
2012;(22):3369-3371,后插2
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a sensitive and specific method for quantitative detection of Hantavirus RNA in serum of patients with hemorrhagic fever with renal syndrome(HFRS)by means of multiple TaqMan-MGB fluorescence quantitative RT-PCR.Methods 96 copies of HFRS have been selected as the test object patients serum,according to hantaan viruses(HV)strains sequence to design primer and TaqManTM probe,through the preparation of genomic DNA primers concentration,probe concentration and other conditions for optimization,the initial formation of HV virus multiple TaqMan-MGB fluorescence quantitative RT-PCR fast detection methods.Results Through the optimization laboratory conditions were after muhiple TaqMan-MGB fluorescence quantitative RT-polymerase chain reaction(PCR)are the most iueal reaction conditions,set up the rapid detection of HV virus method,test process process for 1.5 hours.Conclusion Multiple TaqMan-MGB fluorescence quantitative RT-PCR has high sensitivity,high specific characteristics,detection of short time,improve the detection of the hantaan viruses,help to diagnosis.
