Study on effects of G-CSF and AMD3100 on proliferation, migration and adhesion of rat mesenchymal stem cells
10.3760/cma.j.issn.1673-4181.2012.06.006
- VernacularTitle:G-CSF、AMD3100对大鼠间充质干细胞增殖、迁移和黏附能力影响的研究
- Author:
Hui DONG
;
Qin YU
;
Lizhen LIU
;
Yan WANG
;
Xiaobo XUAN
;
Biao WANG
;
Wei SHAN
;
Liping ZHOU
;
Wei LIU
- Publication Type:Journal Article
- Keywords:
Granulocyte colony-stimulating factor;
AMD3100;
Mesenchymal stem cells;
Proliferation;
Migration;
Adhesion
- From:
International Journal of Biomedical Engineering
2012;(6):343-345,349,后插6
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects ofgranulocyte colony-stimulating factor(G-CSF) and AMD3100 on the proliferation,migration and adhesion of mesenchymal stem cells (MSCs).Methods The proliferation,migration and adhesion of MSCs were detected by MTT chromometry,transwell and adhesion test.Results When the concentration of G-CSF was 200 μg/L and AMD3100 was 0.5 mg/L,the proliferation and migration of MSCs were the strongest.When the concentration of G-CSF was 200 μg/L and AMD3100 was 0 mg/L,the adhesion of MSCs was the strongest.Conclusion The proliferation,migration,adhesion of MSCs are promoted by G-CSF,and inhibited by AMD3100.
