Effects of intracellular magnetic labeling of stem cells with superparamagnetic iron oxide on the differentiation capability into hepatocyte-like cells
10.3760/cma.j.issn.1673-4181.2012.06.005
- VernacularTitle:超顺磁氧化铁标记对大鼠脂肪干细胞向肝样细胞诱导分化的影响
- Author:
Yan LI
;
Zaiyi LIU
;
Changhong LIANG
;
Jiening ZHU
;
Xiaohong LI
;
Jialiang LIANG
- Publication Type:Journal Article
- Keywords:
Superparamagnetic iron oxide;
Adipose-derived stem cells;
Hepatocyte-like cells;
Labeling
- From:
International Journal of Biomedical Engineering
2012;(6):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of intracellular magnetic labeling of stem cells with superparamagnetic iron oxide (SPIO) on the cell differentiation capability into hepatocyte-like cells.Methods Adiposederived stem cells (ADSCs) were obtained from the inguinal fat tissue of Sprague-Dawley rats.ADSCs were labeled with co-incubation of poly-l-lysine (PLL) and SPIO (25 μg/mL).Intracellular iron uptake was analyzed qualitatively with light microscopy.The viability of ADSCs was evaluated with trypan blue staining.SPIO-labeled and unlabled ADSCs were subjected to differentiate into hepatocyte-like cells with hepatocyte growth factor (HGF).Liver marker gene such as albumin (ALB) was analyzed by RT-PCR.The cell viability between the labeled cell group and unlabeled cell group adopted two independent sample t-Test.Results Light microscopy results revealed intracytoplasmic iron uptake and nearly 100％ of cell labeling efficiency.SPIO-labeled ADSCs had an unaltered viability as compared with unlabeled ADSCs (P＞0.05).After induction,glycogen storage within cytoplasm can be found in the two group on 14 d and 21 d,and the cells with positive staining increased on day 21.The two groups both express the ALB on 14 d and 21 d,which expressed higher on 21 d.Conclusion Intracellular magnetic labeling with SPIO did not affect the viability and capability of ADSCs to differentiate into hepatocyte-like cells.
