Preparation and drug-loading property of phosphatidyl polyethyleneglycol monomethyl ether liposome
10.3760/cma.j.issn.1673-4181.2012.06.002
- VernacularTitle:磷脂酰聚乙二醇单甲醚脂质体的制备及其载药性能
- Author:
Li WU
;
Jing CHEN
;
Tianjun LIU
- Publication Type:Journal Article
- Keywords:
Phosphatidyl polyethyleneglycol monomethyl ether;
Liposome;
Long circulation
- From:
International Journal of Biomedical Engineering
2012;(6):325-328,后插3
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop liposome loaded with paclitaxel for the treatment of lung cancer,novel phosphatidyl polyethyleneglycol monomethyl ether liposomes were prepared and studied.Methods A series of phosphatidyl polyethyleneglycol monomethyl ether liposomes were synthesized.Liposomes with or without drug were prepared by ultrasound.The entrapment efficiency and size of drug loaded liposomes were evaluated.The influence of liposomes to A549 lung cancer cell was investigated by MTT method.The cell uptake of liposome was observed by laser confocal scan microscope.Results The liposome loaded with paclitaxel had an entrapment efficiency of 83％ and size of 100～200 nm.The MTT results showed phosphatidyl polyethyleneglycol monomethyl ether liposomes loaded paclitaxel have inhibition effect to A549 cells with long circulation,while liposomes without paclitaxel have not inhibition effect.Conclusion Drug loaded polyethyleneglycol monomethyl ether liposomes have sustained delivery to paclitaxel.They have potential application in the therapy of cancer.
