Obstructive jaundice internal and external biliary drainage' s effects on the levels of TNF-α, No and iNOS
10.3760/cma.j.issn.1673-4203.2013.02.006
- VernacularTitle:内外引流对梗阻性黄疸TNF-α、NO和iNOS的影响
- Author:
Xiaojiang LUO
;
Peijian ZHANG
;
Yongjun JIANG
;
Jiafeng ZHANG
- Publication Type:Journal Article
- Keywords:
Jaundice,obstructive;
Tumor necrosis factor-alpha;
Nitric oxide;
Nitric oxide synthase;
Gastric mucosa
- From:
International Journal of Surgery
2013;(2):92-94,封3
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the different effects of internal and external biliary drainage on the levels of TNF-α,NO in blood serum and NO,inducible nitric oxide synthase(iNOS) in gastric mucosa with obstructive jaundice and explore the mechanism of gastric mucosa damage and compare the quality of internal and external drainage.Methods To establish the animal model:one hundred male adult Sprague-Dawley rats were randomly assigned to five groups:obstructive jaundice for 7 days group (OJ-7 d group),obstructive jaundice for 14 days (OJ-14 d group),internal biliary drainage group(ID group),external drainage group(ED group)and sham operation group(SH group).The OJ-7 group rats were executed 7 days after the first operation,the rest four groups were executed 7 days after the secondary surgery,leaving serum and gastric mucosa.Tested the levels of INF-α,NO in blood serum and NO,iNOS in gastric mucosa and did pathological biopsy of gastric mucosa.Results The levels of TNF-α,NO in blood serum and NO,iNOS in gastric mucosa significantly increased in OJ-7 group.The levels of TNF-α,NO in blood serum and NO,iNOS in gastric mucosa continued to rise in OJ-14 group,and was significantly higher than OJ-7 group,the gastric mucosa was obviously impaired.The levels of TNF-α,Internal drainage can significantly NO in blood serum and NO,iNOS in gastric mucosa were significantly lower in ID group,gastric mucosal damage significantly reduced.Conclusions After internal drainage,iNOS expression was inhited in gastric mucosal,NO production was significantly reduced,led to protect the gastric mucosa,which provide experimental basis for choice in OJ clinical treatment.
