Relation between perfusion speed and acute separation of myocardial cells in New Zealand rabbits

VernacularTitle:灌流速度与新西兰兔心肌细胞急性分离的关系
Author: Xiaofeng XU ; Weihua LI ; Zhengrong HUANG
Publication Type:Journal Article
Keywords: Cell separation; Myocardium; Rabbits
From: Chinese Journal of cardiovascular Rehabilitation Medicine 2013;22(1):40-43
CountryChina
Language:English
Abstract: Objective: To establish a simple, stable and high success rate separation method of myocardial cells in New Zealand rabbits. Methods: Compared influence between perfusion speed of 50 r/min (8 ml/min) and 65 r/min (11 ml/min) of Langendorff perfusion device on cell separation during separation course of myocardial cells. Results: Compared with perfusion speed of 50 r/min, there was significant decrease in time to obtain cells [(33±9.10) min vs. (19.29±2.73) min], and significant increase in success rate (50% vs. 91%) and survival rate [(38.26±2.63) % vs. (85.71±2.21) %] by perfusion speed of 65 r/min, P<0.01 all. Conclusion: Perfusion speed is an important factor that affects acute separation of myocardial cells in New Zealand rabbits, and 65r/min perfusion speed may be more stable and successful to obtain single myocardial cell.
Full text:P020130514554585779709.doc