Effect of lipid raft on the growth of cervical cancer cells
10.3760cma.j.issn.1006-9801.2012.05.003
- VernacularTitle:脂筏对子宫颈癌细胞生长的影响
- Author:
Yanxiang CHENG
;
Hong XU
;
Limei ZHOU
;
Jinling HUANG
;
Bingshu LI
;
Min HU
- Publication Type:Journal Article
- Keywords:
Lipid raft;
Uterine cervical neoplasms;
NADPH oxidase;
Hypoxia-inducible factor 1,alpha subunit
- From:
Cancer Research and Clinic
2012;24(5):295-296,303
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of lipid raft on cervical cancer cell growth and its mechanisms Methods HeLa cells in logarithmic phase were divided into three groups including control group, lipid raft interference agent group,and NADPH oxidase inhibitors group.Cells were treated with fre sh medium,3 μmol/L Apocynin and 1 mmol/L M-beta CD, respectively, for 24 h.Cell survival rate was detected using the MTT method, and the HIF-1α level was examined by Western-blot. Results Cell growths of the lipid raft interference agent group and NADPH oxidase inhibitors group were significantly slower than control group,(0.612±0.051 vs 0.984±0.034,0.591 ±0.074 vs 0.984±0.034,t=4.062,P＜0.05).HIF-1α expression in the lipid raft interference agent group and NADPH oxidase inhibitors group was also significantly reduced compared with control group (1.79±0.14 vs 2.56±0.22 and 1.54±0.12 vs 2.56±0.22) and the difference was significant (t=2.423,P＜0.05). Conclusion Lipid raft-NADPH oxidase pathway may activate HIF-1α and downstream protocarcinogenic gene expression to promote the growth of cervical cancer cells. The inhibitors of lipid raft and NADPH oxidase may become a new research direction for cervical cancer chemotherapy.
