Effect of TGF-β1 and CTGF on skeletal muscle fibrosis in nerve compression

VernacularTitle:TGF-β1和CTGF在周围神经卡压后骨骼肌纤维化中的作用
Author: Zhongwei JIA ; Jianghai CHEN ; Yanhua CHEN ; Rui HU ; Fanbin MENG ; Yuxiong WENG ; Zhenbing CHEN
Publication Type:Journal Article
Keywords: Transforming growth factor-β1; Connective tissue growth factor; Type Ⅰ collagen; Peripheral nerve; Skeletal muscle; Fibrosis
From: Chinese Journal of Microsurgery 2012;35(2):135-138,后插5
CountryChina
Language:Chinese
Abstract: ObjectiveTo investigate expression of TGF-β1,CTGF and collagen deposition in skeletal muscle during chronic entrapment of peripheral nerve. MethodsFifty rats were separated into two groups,control group and experimental group. At different time points after operation, the right gastrocnemius of 5rats from each group were collected for further analysis such as HE, Masson stain, immunohistochemical staining,RT-PCR and Western-blot. Results It was observed that axon degeneration occurred during chronic nerve entrapment,and which was in line with reports from other groups.Moreover,it had been demonstrated that after nerve entrapment,skeletal muscles may form fibrosis and degeneration consequently.Within this pathological procedure,expression of TGF-β1. CTGF and deposition of collagenⅠ changed rapidly when compared with control group.ConclusionOverall,these results indicated that these factors may be important during skeletal muscle degeneration after chronic nerve entrapment.