SFRP2 gene promoter hypermethylation and its clinicopathologic significance in colorectal carcinoma
10.3760/cma.j.issn.1673-4203.2012.08.007
- VernacularTitle:SFRP2基因超甲基化与结直肠癌临床病理关系的研究
- Author:
Haifeng YU
;
Daorong WANG
;
Yun ZHANG
;
Chao JIANG
;
Sujun ZHOU
;
Guoqiang ZHANG
;
Yongkun LI
;
Jie CHEN
;
Dong TANG
;
Tianzhou CHA
- Publication Type:Journal Article
- Keywords:
Colorectal neoplasms;
Tumor-infiltrating;
Neoplasm metastasis;
Methylation;
Polymerase chain reaction;
Pathology,clinical
- From:
International Journal of Surgery
2012;39(8):526-529,封3
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the differential level of hypermethylated SFRP2 gene in colorectal cancers andadjacent nontumorous tissues,to analyze the relation of SFRP2 gene promoter hypermethylation status and its clinicopathologic significance in colorectal carcinoma,and to study the relationship between the level of hypermethylated SFRP2 and the invasion and metastasis of colorectal carcinoma.Methods Real-time quantitative PCR technique was performed to analyze the level of hypermethylated SFRP2 gene promoter in colorectal cancers and adjacent nontumorous tissues taken from 30 colorectal cancer patients.The relation of the level of hypermethylated SFRP2 gene promoter and its clinicopathologic features of colorectal cancers was analyzed.Results SFRP2 gene promoter hypermethylation occurred in both the tumor tissues and the adjacent tissues.The level of SFRP2 gene promoter hypermethylation was significantly higher in the patients with TNM Ⅲ and Ⅳ (7.24 ± 1.13)than in patients with TNM Ⅰ and Ⅱ ( 5.92 ± 0.97 ) ( P ＜ 0.05 ).The level of SFRP2 gene promoter hypermethylation was significantly higher in the patients with lower differentiation(7.31 ± 1.11 ) than in patients with higher differentiation (6.23 ± 1.03) ( P ＜ 0.05 ).No significant association was found between the level of SFRP2 gene promoter hypermethylation and the status of gender,age.tumor location and tumor size ( P ＞ 0.05 ).Conclusions SFRP2 gene hypermethylation in tissues may be a useful objective parameter for the malignant level,invasion,metastasis,recurrence and prognosis of the colorectal cancer.
