The effect of let-7b on melanoma cell proliferation and aerobic glycolysis

VernacularTitle:Let-7b对人黑色素瘤细胞增殖及有氧糖酵解的影响
Author: Jianda ZHOU ; Ming LI ; Wanmeng LI ; Ming ZHOU ; Chengqun LUO ; Chaoqi YIN ; Tiefu CHEN ; Ping LI ; Jia CHEN ; Bimei JIANG ; Haihang LIU ; Rui LIU ; Dan XU ; Huiqing XIE
Publication Type:Journal Article
Keywords: MicroRNAs/pharmacology; Melanoma/pathology/physiopathology/drug therapy; Glycolysis
From: Journal of Chinese Physician 2012;14(5):577-579,584
CountryChina
Language:Chinese
Abstract: Objective To study the influence of let-7b on cell proliferation and aerobic glycolysis of human melanoma cell A375.Methods Transfect A375 cell line with hsa-let-7b oligonucleotide or antisense.Glucose and lactate in medium were determined by spectrophotometry at 24 h and 48 h time point after transfection.The cell proliferation was determined by methylthiazol tetrazolium (MTT) assay.Results Over expression of let-7b in melanoma cell reduced cell proliferation notably,compared to the other groups by MTT(P ＜0.05).However,the glucose consumption and lactate production differences were not observed during 24 h or 48 h ( P ＞ 0.05 ),the blank control group transformed about 57％ and 43％ glucose to lactate during 24 h and 48 h.Conclusions Melanoma cell line A375 has notably aerobic glycolysis hallmark,let-7b could inhibit proliferation of melanoma cell line A375,but it may has no influence on glucose metabolism.