Effects of different types of cell culture dishes on fluorescent image with confocal microscope
10.3760/cma.j.issn.1673-4181.2012.03.012
- VernacularTitle:不同细胞培养皿对激光共聚焦显微镜成像效果的影响
- Author:
Liyun PANG
;
Hai WANG
;
Huanlai LIU
;
Yuqi XU
;
Dunwan ZHU
- Publication Type:Journal Article
- Keywords:
Cell culture dish;
Confocal microscope;
Immunofluorescence
- From:
International Journal of Biomedical Engineering
2012;35(3):177-180,后插5,后插6
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of different thickness of cell culture dishes on fluorescent image with confocal microscope.Methods The fluorescent staining experiments of live cells and fixed cells were used to determine the differences among three dishes with different thickness coverslips of 0.085～0.13 mm,0.13～0.16 mm and 0.16～0.19 mm,while the cell appearance,fluorescence lightness and mean of fluorescence intensity were studied with confocal microscope.Results Demonstrated by the results of cytoskeleton staining experiments,the dish with 0.13～0.16 mm thickness coverslip was the best choice for confocal microscope,the dish with 0.16～0.19 mm thickness coverslip was the second one,the dish with 0.16～0.19 mmthickness coverslip was the last one.ConclusionThe dish with 0.13～0.16 mm thickness coverslip is the best choice for confocal microscope.On this type of dish,the cytoskeleton is unfolding and clear after staining.The intensity of fluorescence is the strongest,and the imaging effect is the best.
