Construction and verification of lentivirus vector expressing mouse notch intracellular domain
10.3760/cma.j.issn.1673-4181.2012.03.002
- VernacularTitle:表达小鼠Notch1胞内段基因慢病毒载体的构建及鉴定
- Author:
Quan TANG
;
Bing YANG
;
Hui ZHOU
;
Fujun YANG
;
Rong DUAN
;
Tiqiang FAN
;
Ying HAN
;
Xiaodong ZHANG
;
Yuanming SUN
- Publication Type:Journal Article
- Keywords:
Lentiviral;
Notch intracellular domain;
Notch1
- From:
International Journal of Biomedical Engineering
2012;35(3):133-136,后插2
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveNotch signaling is highly conservative in evolution and plays an important role in cell's proliferation and differentiation.Construction of tentiviral vector containing Notch intracellular domain (NICD) would lay the foundation for the study of Notch signaling.MethodsTotal RNA was extracted from the myeloid tissue of C57BL/6 mice.cDNA was composed via reverse transcription.NICD sequence was obtained by PCR and recombined into lentiviral vector.Lentiviral vector with NICD was infected into target HEK293T cell.Real-time PCR and Western blot were used to examine NICD expression in HEK293T cell.ResultsNICD expression increased in HEK293T cells.ConclusionThe successful construction of lentiviral vector involving mice NICD expression provides the foundation for the future study.
