Autophagic cell death in mouse melanoma B16 cells induced by TNF-α
10.3760/cma.j.issn.1673-4181.2012.03.006
- VernacularTitle:TNF-α诱导B16细胞自体吞噬的作用研究
- Author:
Wenhui YAN
;
Liping ZHAO
;
Haixuan QIAO
- Publication Type:Journal Article
- Keywords:
Tumor necrosis factor-α;
B16 cells;
Autophagy
- From:
International Journal of Biomedical Engineering
2012;35(3):151-154,后插3
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo analyze the autophagy of mouse melanoma B16 cells induced by TNF-ct.MethodsMouse melanoma B 16 cells treated with TNF-α were used in this study.The expression levels of LC3- Ⅱand Beclinl were measured by western blot and mRNA levels of autophagy related gene atg5,atg7 and atgl2 were measured by reverse transcription polymerase chain reaction (RT-PCR) after TNF-α treatment.ResultsThe viability of B16 cells were obviously inhibited after incubation with TNF-α.The expression levels of autophagy related protein LC3- Ⅱ and Beclinl were elevated in TNF-α treated B16 cells compared with in control B16 cells.The mRNA expression levels of autophagy related gene Atg5,atg7 and atg12 showed consistent up regulation in TNF-a treated B16 cells compared with in control B16 cells.ConclusionTNF-α can induce autophagic cell death in B16 cells.
