Study of the proliferation ability of human degenerate nucleus pulposus and annular fibrosus cell in vitro
10.3760/cma.j.issn.1673-4181.2012.02.012
- VernacularTitle:体外培养人退变髓核与纤维环细胞增殖能力的研究
- Author:
Yan LIU
;
Xiaolei SUN
;
Zhao YANG
;
Shuang LI
;
Xinlong MA
- Publication Type:Journal Article
- Keywords:
Intervertebral discs degeneration;
Nucleus pulposus cells;
Annulus fibrosus cells;
Proliferation index
- From:
International Journal of Biomedical Engineering
2012;35(2):112-115,后插8
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the proliferation of human degenerate nucleus pulposus and annular fibrosus cell in vitro and compare the different biological behavior between the two kinds of cell after degeneration,and provide the new theoretical basis for the prevention and treatment of degenerative disc disease.MethodsThe samples of intervertebral disc tissue were collected from patients with lumbar disc herniation.The degree of degeneration was assessed by the pathological diagnosis and degenerate nucleus pulposus and annular fibrosus cell were cultured by enzymatic digestion and identified.In each case,the control groups of the nucleus pulposus cells and annulus fibrosus cells were cultured to the fifth generation.The inoculation density of cells was 1×105.The cell morphology of each generation was observed,while the proliferation of cells was detected by flow cytometry after 48h culture with the same conditions.ResultsThe degenerate nucleus pulposus cell and annular fibrosus cell were in good condition in vitro.The percentages of S phase cell and proliferation index (PI) were both on the rise with the subculture.The PI of nucleus pulposus cells reached the peak in the 3rd generation; The PI of annulus cells was the highest in the generation 5.The proliferation activity of degenerate nucleus pulposus cell in 2～4 generations was higher than the degenerate annular fibrosus cell within the same generations (P＜0.05).ConclusionDifferent proliferative characteristics of the degenerate nucleus pulposus and annular fibrosus cell confirmed that the disc degeneration is reversible.The response mechanisms to the degenerate micro-environment are completely different in vivo and affects the entire disc degeneration progress.
