The effect of Ghrelin on activation of UCP3 and phosphorylation of ACC under the high fat environment in rat myoblast

VernacularTitle:Ghrelin对高脂环境下成肌细胞UCP3活化及ACC磷酸化的影响
Author: Lingling HAN ; Yuxia WANG ; Jia LI ; Guoliang LIU
Publication Type:Journal Article
Keywords: Ghrelin/PD; Hyperlipidemias/ME; Myoblasts/ME; Acetyl-CoA carboxylase/ME; Proteins/ME; Uncoupling agents
From: Journal of Chinese Physician 2012;14(2):163-165,169
CountryChina
Language:Chinese
Abstract: ObjectiveTo study the effect of Ghrelin on activation of UCP3 and phosphorylation of ACC under the high fat environment in rat myoblast,and to support the study on prevention of type 2 diabetes.MethodsThe rat myoblast were exposed to 0.3 mmol/L palmitic acid for 12 h,at the same time,10-11 mol/L,10-9 mol/L,10-7 mol/L Ghrelin were added.The expression of UCP3 mRNA was determined by RT-PCR.The protein levels of UCP3 and p-ACC were measured by western blotting.ResultsCompared with the control group,UCP3 mRNA and the protein levels of UCP3 and p-ACC were obviously decreased in 0.3 mmol/L PA group(UCP3 mRNA:P =0.000,UCP3 protein levels:P =0.000,p-ACC protein levels:P =0.003).Compared with the 0.3 mmol/L PA group,with the increasing concentration of Ghrelin,UCP3 mRNA and the protein levels of UCP3 and p-ACC were elevated.The effects of 10-7 mol/L Ghrelin were significant(UCP3 mRNA:P =0.017,UCP3 protein levels:P =0.000,p-ACC protein levels:P =0.007).ConclusionsGhrelin could enhance activation of UCP3 and phosphorylation of ACC under high fat environment in rat myoblast.