Changes of gene methylation profile in malignant transformation of immortalized human bronchial epithelial cell line induced by alpha-particle irradiation
10.3760/cma.j.issn.0254-5098.2011.04.011
- VernacularTitle:α粒子诱发永生化人支气管上皮细胞恶性转化过程中DNA甲基化的变化
- Author:
Yingchun HU
;
Zhongmin CHEN
;
Qiaodan ZHOU
;
Boqiang SONG
;
Gang LI
;
Dechang WU
;
Yanying HUO
- Publication Type:Journal Article
- Keywords:
α particle;
Methylation microarray;
Malignant transformation;
Immortalized human bronchial epithelial cell line BEP2D
- From:
Chinese Journal of Radiological Medicine and Protection
2011;31(4):420-424,432
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the changes of DNA methylation profile in the process of malignant transformation of BEP2D cell induced by α particles.Methods The genomic DNAs were isolated from the malignant transformation BERP35T4 cells and immortalized human bronchial epithelial cell line BEP2D.Genomic DNAs were digested by MseI and ligated of PCR linkers.Methylated DNAs were digested by BstUI and amplified by PCR.The methylated DNA probes were prepared by labeling with Cy3 and Cy5 fluorescence dyes individually and hybridized to the methylation CpG-Island microarray.The hybridization results were scanned and analyzed.Intensity values were quality controlled and normalized.The normalized data were used to identify the differentially expressed genes based on a 1.5 fold difference of the expression level.Results There were 16 genes which showed changes of methylation level in malignant transformation BERP35T4 cells, 9 of them were hypermethylation and 7 were hypomethylation.These genes were including the SKIP gene, PPP3CC gene, MAP2K6 gene, KIR2DL1 gene, KIR2DL4 gene, KIR3DP1 gene, ZNF493 gene, ZNF100 gene, NKX2-5 gene, TFAP2D gene, DR1 gene, KCNJ16 gene, CCDC18 gene, FNBP1L gene, IRX4 gene, EPB41L3 gene, TCP10 gene and so on.Conclusions The DNA methylation might have effects on ionizing radiation drived tumorigenesis.
