Establishment of the limited cell line of skin fibroblast and their differentiation to hepatic cells

VernacularTitle:皮肤成纤维细胞体外长期培养及肝向分化研究
Author: Zhengyan ZHU ; Zhi DU ; Ying LUO ; Jiao LI ; Linlin SUN ; Peng WANG ; Tong LIU ; Yingtang GAO
Publication Type:Journal Article
Keywords: Fibroblasts; Differentiation; Hepatocyte-like cells
From: International Journal of Biomedical Engineering 2011;34(5):284-287,292
CountryChina
Language:Chinese
Abstract: Objective To establish the long-term culture system for fetal skin fibroblast by performing long time in vitro cultivation of the cells,and study the potential of its differentiation to hepatocytes.Methods Fibroblast was isolated from human fetus skin tissue.Surface phenotypes of cells were detected by ICC and FCM,and biological characteristics were analyzed by the karyotype analysis and soft agar colony formation observation.ALB、CK18、CK19 were detected by ICC,glycogen stain by PAS,AFP and ALB mRNA by RT-PCR after P3～30cells were induced differentiation by cytokines of HGF,FGF4 and OSM.Results CD29,CD49f,HLA- Ⅰ and CD 105 were highly expressed while CD90 hardly in skin fibroblast.The rate of induced differentiation of fibroblast into hepatocyte-like cells was approximately 5％.The cells could be cultured in vitro for almost 50 passages with normal karyotype and no oncogenic and immortalized characteristics.Conclsion The skin fibroblast possesses the characteristic of mesenchymal stem cell and can be induced into hepatocyte-like cell in vitro.