Correlation between drug-resistant genes and mobile genetic elements in multi-drug resistant Escherichia coli
10.3760/cma.j.issn.1674-2397.2011.05.010
- VernacularTitle:大肠埃希菌耐药相关基因和可移动遗传元件指标与样本聚类分析
- Author:
Dongbiao HUANG
;
Sulan XU
;
Maoliang ZHOU
;
Xiaoyan HU
;
Yongjun MA
;
Haishen. KONG
- Publication Type:Journal Article
- Keywords:
Escherichia coli;
Drug resistance;
Beta-lactams;
Aminoglycosides;
Mobile genetic element;
Index cluster analysis;
Sample cluster analysis
- From:
Chinese Journal of Clinical Infectious Diseases
2011;04(5):288-291,316
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlation between drug-resistant genes and mobile genetic elements in multi-drug resistant Escherichia coli,and to explore phylogeny among the strains.MethodsTotally 20 strains of multi-drug resistant Escherichia coli were collected from Pan' an Hospital,Zhejiang Province during June 2009 and June 2010.Beta-lactam-resistance genes,aminoglycoside-resistance genes,genetic markers of mobile genetic elements were analyzed by PCR.Index and sample cluster analysis were performed on above results. Results In 20 strains of Escherichia coli,4 kinds of beta-lactamresistance genes,4 kinds of aminoglycoside-resistance genes,and 5 kinds of genetic markers of mobile genetic elements were detected.Index cluster analysis showed that correlation existed between resistance genes TEM,CTX-M-1,aadA5 and mobile genetic elements traA,IS26,ISEcpl; and correlation also existed between resistance genes OXA-1,aac(6′)-Ⅰ b,ant(3)-Ⅰ,rmtB and mobile genetic elements trbC,IS903.Sample cluster analysis showed that this group of Escherichia coli could be divided into 2 groups which were genetically different.ConclusionsDrug-resistant genes in multi-drug resistant Escherichia coli are correlated with mobile genetic elements.Sample cluster analysis can reveal phylogeny among the strains,which is important for hospital infection control.
