The expression of Hsc70 in synovial membranes and blood of patients with rheumatoid arthritis
10.3760/cma.j.issn.1007-7480.2012.11.006
- VernacularTitle:结构型热休克蛋白70在类风湿关节炎患者滑膜组织和血液中表达的研究
- Author:
Qingsong MENG
;
Yan ZHAO
;
Shui SUN
;
Xiaotian CHANG
;
Wenbo LIU
;
Xinfeng YAN
- Publication Type:Journal Article
- Keywords:
Arthritis,rheumatoid;
Heat-shock proteins70;
Immunoassay
- From:
Chinese Journal of Rheumatology
2012;16(11):741-744,后插1
- CountryChina
- Language:Chinese
-
Abstract:
Objective The present study investigated the expression of heat shock cognate protein 70 (Hsc70) in the synovial tissues and blood samples of patients with rheumatoid arthritis (RA) to determine the pathological role of this protein in the pathogenesis of the disease.Methods The expression of Hsc70 in synovial membranes was quantitatively analyzed by immunohistochemistry,real-time quantitative PCR and western blotting.The samples from osteoarthritis (OA) and ankylosing spondylitis (AS) were used as controls.The levels of Hsc70 in blood of patients with RA were determined using enzyme linked immunosorbent assay (ELISA) with the samples of the healthy subjects as controls.Statistical analysis was conducted with one-way ANOVA,LSD test and Spearmen's correlation.Results Immunohistochemistry showed that Hsc70 had significantly increased expression in synovial tissues of RA than in the samples of OA and AS.Real-time PCR and western blotting confirmed the above findings.ELISA detected significantly elevated level of Hsc70 in blood of patients with RA as compared with samples from the controls (P＜0.01).Conclusion The study suggests that the up-regulation of Hsc70 may be involved in the pathogenic process of RA.
