Transforming growth factor-β1 generates epithelial-to-mesenchymal transition and promote CD44 expression in SGC7901 cells
10.3760/cma.j.issn.1673-4203.2012.11.009
- VernacularTitle:TGF-β1诱导胃癌细胞上皮间质转化及其上调CD44表达的实验研究
- Author:
Cheng CAI
;
Jiwei YU
;
Jugang WU
;
Ruiqi LU
;
Xiaochun NI
;
Shoulian WANG
;
Bojian JIANG
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
Carcinoma;
Antigens,CD44;
Transforming growth factor-β1;
Epithelial mesenchymal transition
- From:
International Journal of Surgery
2012;39(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the biological effect of epithelial-to-mesenchymal transition (EMT) under the treatment of transforming growth factor (TGF)-β1 on the human gastric cancer cell line SGC7901 in vitro,and to observe whether the TGF-β1 can generate the tumor initiating cells ability in SGC7901 or not.Methods SGC7901 cells were cultured with TGF-β1.The morphological change was observed.The effect on proliferation of SGC7901 cells was detected by CCK-8.The invasion assay was used to investigate the motility and the invasion ability of SGC7901 cells.Immuofluorescence was used to detect the expression of E-cadherin and N-cadherin.The mRNA and protein's expression levels of EMT-related factors and CD44 were analyzed by RT-PCR and Western blotting respectively.Results TGF-β1 induced morphological alterations from epithelial to mesenchymal cells.The proliferation of SGC7901 cells was inhibited,and the ability of motility and invasion of SGC7901 cells were greatly enhanced after being treated with TGF-β1.RT-PCR and Western blotting showed that the expression of Snail (P ＜ 0.05),N-cadherin (P ＜ 0.05) and CD44 (P ＜ 0.05) were significantly increased while the expression of E-cadherin was decreased (P ＜ 0.05).Conclusions TGF-β1 can generate the EMT.The CD44 expression was up-regulated.TGF-β1 can inhibit the proliferation and promote the motility and invasion ability of SGC7901 cells.
